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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54783 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HSPA8 Antibody / HSC70
- Antibody type
- Polyclonal
- Description
- This highly specific HSPA8 antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the HSPA8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A431, 2) human HeLa, 3) mouse NIH 3T3 and 4) rat H-4-II-E cell lysate with HSPA8 antibody. Expected molecular weight: 70-73 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human A2058 cells with HSPA8 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with HSPA8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with HSPA8 antibody; Blue=isotype control, Green= HSPA8 antibody.
