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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MA1-10891 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSC70 Monoclonal Antibody (BB70)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-10891 detects Heat Shock Protein 70/Heat Shock Cognate Protein 70 in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, yeast, fish and Xenopus samples. MA1-10891 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, MA1-10891 is specific for the ~72 kDa Heat Shock Protein 70/Heat Shock Cognate Protein 70 protein. MA1-10891 immunogen is chicken Hsp70/Hsp90 complex.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BB70
- Vial size
- 100 µg
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.
Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.
Prapapanich V, Chen S, Smith DF
Molecular and cellular biology 1998 Feb;18(2):944-52
Molecular and cellular biology 1998 Feb;18(2):944-52
Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.
Prapapanich V, Chen S, Smith DF
Molecular and cellular biology 1998 Feb;18(2):944-52
Molecular and cellular biology 1998 Feb;18(2):944-52
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of rat liver and fish ventricle lysates using Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product # MA1-10891). Whole tissue extracts from rat liver (20 µg) and Chaenocephalus aceratus and Notothenia coriiceps ventricles (200 µg) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Following transfer, membranes were blocked with 5% milk in PBST and probed with Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product # MA1-10891) at 1:2000 dilution followed by donkey anti-mouse IgG secondary antibody (Product # PA1-28748) at 1:5000 dilution and a chemiluminescent substrate. Data courtesy of the Innovators Program
