Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-24624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSC70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, hamster, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A Proteomic Study Suggests Stress Granules as New Potential Actors in Radiation-Induced Bystander Effects.
Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors.
Tudor M, Gilbert A, Lepleux C, Temelie M, Hem S, Armengaud J, Brotin E, Haghdoost S, Savu D, Chevalier F
International journal of molecular sciences 2021 Jul 26;22(15)
International journal of molecular sciences 2021 Jul 26;22(15)
Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors.
Nuss JE, Kehn-Hall K, Benedict A, Costantino J, Ward M, Peyser BD, Retterer CJ, Tressler LE, Wanner LM, McGovern HF, Zaidi A, Anthony SM, Kota KP, Bavari S, Hakami RM
PloS one 2014;9(5):e93483
PloS one 2014;9(5):e93483
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a HSPA8/Heat Shock 70 Protein 8 polyclonal antibody (Product # PA5-24624) in HeLa cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPA8 (arrow) using a HSPA8/Heat Shock 70 Protein 8 polyclonal antibody (Product # PA5-24624) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSPA8 gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), PC3 (Lane 2), CaCO-2 (Lane 3), Hep G2 (Lane 4), K-562 (Lane 5), Raji (Lane 6), SH-SY5Y (Lane 7), HL-60 (Lane 8) and A431 (Lane 9). The blot was probed with Anti-HSC70 Polyclonal Antibody (Product # PA5-24624, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 70 kDa band corresponding to HSC70 was observed across all the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HSC70 was achieved by transfecting HeLa with HSC70 specific siRNAs (Silencer® select Product # s6987). Western blot analysis (Fig. a) was performed using whole cell extracts from the HSC70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HSC70 Polyclonal Antibody (Product # PA5-24624, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HSC70.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A2058 cells using a HSPA8/Heat Shock 70 Protein 8 polyclonal antibody (Product # PA5-24624) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a HSPA8/Heat Shock 70 Protein 8 polyclonal antibody (Product # PA5-24624), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a HSPA8/Heat Shock 70 Protein 8 polyclonal antibody (Product # PA5-24624) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Western blotting analysis of cyclophilin A, thioredoxin, alpha-enolase, RPLP0, HSC70, HSPA9, and CCT3 in a whole-cell extracts from T/C-28A2 bystander cells receiving the conditioned medium of low-dose irradiated chondrosarcoma cells (0.1 Gy) or non-irradiated chondrosarcoma cells (CTR).