- PA5-29221 - Invitrogen Antibodies HSPA8 antibody

Shah AP, Johnson MD, Fu X, Boersma GJ, Shah M, Wolfgang MJ, Tamashiro KL, Baraban JM
International journal of obesity (2005) 2020 Jan;44(1):254-266

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), PC3 (Lane 2), K-562 (Lane 3), Raji (Lane 4) and SH-SY5Y (Lane 5). The blot was probed with Anti-HSC70 Polyclonal Antibody (Product # PA5-29221, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 70 kDa band corresponding to HSC70 was observed across all the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HSC70 Polyclonal Antibody (Product # PA5-29221). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HeLa. 7.5% SDS PAGE. HSC70 Polyclonal Antibody (Product # PA5-29221) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HSC70 Polyclonal Antibody detects HSC70 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.7.5% SDS-PAGE. HSC70 Polyclonal Antibody (Product # PA5-29221) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HSC70 Polyclonal Antibody (Product # PA5-29221). Sample (30 µg of whole cell lysate). Lane A: Neuro2A. Lane B: GL261. Lane C: C8D30. 7.5% SDS PAGE. HSC70 Polyclonal Antibody (Product # PA5-29221) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of HSC70 was achieved by transfecting HeLa with HSC70 specific siRNAs (Silencer® select Product # s6987). Western blot analysis (Fig. a) was performed using whole cell extracts from the HSC70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HSC70 Polyclonal Antibody (Product # PA5-29221, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HSC70.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: HSC70 Polyclonal Antibody detects HSC70 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSC70 protein stained by HSC70 Polyclonal Antibody (Product # PA5-29221) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody (Product # PA5-29221) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody (Product # PA5-29221) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70 (Product # PA5-29221) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5: Characterization of adipose tissue. A: Representative images of H&E stained eWAT. Scale bars, 100mu. B: Percentage frequency distribution of adipocyte area (left), Friedman test showed a significant difference between the distributions ( P = 0.0042) and correlation between %fat mass and average adipocyte area (in mum 2 ) for WT-LFD ( r = 0.95, P = 0.0139), WT-HFD ( r = 0.85, P = 0.0317) and KO-LFD ( r = 0.83, P = 0.0415) (right). C: Representative bands for trax and translin protein in eWAT (left) and their quantification normalized to heat shock cognate 71 kDa (Hsc70) (right). n = 5, 6 and 6 for the three groups, respectively. Data are expressed as Mean +- SEM. Statistical significance was assessed by Student's t -test, ordinary or Friedman's test followed by Dunn's post-hoc analysis; (white circles/bars = WT-LFD; grey squares/bars = WT-HFD; black triangles = KO-LFD).

PA5-29221