PA1214

antibody from Boster Biological Technology

Targeting: HSPA8 HSC70, HSC71, HSP73, HSPA10

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1214

Provider

Boster Biological Technology

Product name

Polyclonal Anti-HSP70s

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to a sequence at the N-terminal of human HSP70s, identical to the related rat and mouse sequence.

Reactivity

Human, Mouse, Rat

Isotype

IgG

Vial size

100µg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image