ADI-SPA-815-F

antibody from Enzo Life Sciences

Targeting: HSPA8 HSC70, HSC71, HSP73, HSPA10

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ADI-SPA-815-F

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-SPA-815-F, RRID:AB_11181186

Product name

HSC70/HSP73, mAb (1B5)

Antibody type

Monoclonal

Antigen

Other

Description

Protein G affinity purified. Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Isotype

IgG

Antibody clone number

1B5

References

Submitted references

NDR Kinases Are Essential for Somitogenesis and Cardiac Looping during Mouse Embryonic Development.

Schmitz-Rohmer D, Probst S, Yang ZZ, Laurent F, Stadler MB, Zuniga A, Zeller R, Hynx D, Hemmings BA, Hergovich A
PloS one 2015;10(8):e0136566

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K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.

Ferreira JV, Soares AR, Ramalho JS, Pereira P, Girao H
Scientific reports 2015 May 11;5:10210

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Dependence of intracellular and exosomal microRNAs on viral E6/E7 oncogene expression in HPV-positive tumor cells.

Honegger A, Schilling D, Bastian S, Sponagel J, Kuryshev V, Sültmann H, Scheffner M, Hoppe-Seyler K, Hoppe-Seyler F
PLoS pathogens 2015 Mar;11(3):e1004712

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Keratin 8 absence down-regulates colonocyte HMGCS2 and modulates colonic ketogenesis and energy metabolism.

Helenius TO, Misiorek JO, Nyström JH, Fortelius LE, Habtezion A, Liao J, Asghar MN, Zhang H, Azhar S, Omary MB, Toivola DM
Molecular biology of the cell 2015 Jun 15;26(12):2298-310

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STUB1 mutations in autosomal recessive ataxias - evidence for mutation-specific clinical heterogeneity.

Heimdal K, Sanchez-Guixé M, Aukrust I, Bollerslev J, Bruland O, Jablonski GE, Erichsen AK, Gude E, Koht JA, Erdal S, Fiskerstrand T, Haukanes BI, Boman H, Bjørkhaug L, Tallaksen CM, Knappskog PM, Johansson S
Orphanet journal of rare diseases 2014 Sep 26;9:146

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Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat.

Gleixner AM, Pulugulla SH, Pant DB, Posimo JM, Crum TS, Leak RK
Cell and tissue research 2014 Jul;357(1):43-54

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Silencing of human papillomavirus (HPV) E6/E7 oncogene expression affects both the contents and the amounts of extracellular microvesicles released from HPV-positive cancer cells.

Honegger A, Leitz J, Bulkescher J, Hoppe-Seyler K, Hoppe-Seyler F
International journal of cancer 2013 Oct 1;133(7):1631-42

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Basal and stress-induced Hsp70 are modulated by ataxin-3.

Reina CP, Nabet BY, Young PD, Pittman RN
Cell stress & chaperones 2012 Nov;17(6):729-42

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Methylglyoxal alters the function and stability of critical components of the protein quality control.

Bento CF, Marques F, Fernandes R, Pereira P
PloS one 2010 Sep 24;5(9):e13007

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Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens.

Li PP, Itoh N, Watanabe M, Shi Y, Liu P, Yang HJ, Kasamatsu H
Journal of virology 2009 Jan;83(1):37-46

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Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases.

Chen S, Brown IR
Cell stress & chaperones 2007 Spring;12(1):51-8

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Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking.

Lu HA, Sun TX, Matsuzaki T, Yi XH, Eswara J, Bouley R, McKee M, Brown D
The Journal of biological chemistry 2007 Sep 28;282(39):28721-32

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Exogenous androgen treatment delays the stress response following hamster facial nerve injury.

Tetzlaff J, Tanzer L, Jones KJ
Journal of neuroendocrinology 2007 May;19(5):383-9

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Fever-like hyperthermia controls T Lymphocyte persistence by inducing degradation of cellular FLIPshort.

Meinander A, Söderström TS, Kaunisto A, Poukkula M, Sistonen L, Eriksson JE
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3944-53

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Heat-shock protein 90 complexes in resting and thrombin-activated platelets.

Suttitanamongkol S, Polanowska-Grabowska R, Gear AR
Biochemical and biophysical research communications 2002 Sep 13;297(1):129-33

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Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy.

Bailey CK, Andriola IF, Kampinga HH, Merry DE
Human molecular genetics 2002 Mar 1;11(5):515-23

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Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing.

Yamano T, Murata S, Shimbara N, Tanaka N, Chiba T, Tanaka K, Yui K, Udono H
The Journal of experimental medicine 2002 Jul 15;196(2):185-96

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The synthetic peptide P-197 inhibits human T-cell leukemia virus type 1 envelope-mediated syncytium formation by a mechanism that is independent of Hsc70.

Brighty DW, Jassal SR
Journal of virology 2001 Nov;75(21):10472-8

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HSC70 interactions with SV40 viral proteins differ between permissive and nonpermissive mammalian cells.

Sainis L, Angelidis C, Pagoulatos GN, Lazaridis L
Cell stress & chaperones 2000 Apr;5(2):132-8

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) (Prod No. ADI-SPP-751) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) (Prod No. ADI-LYC-3T100) Lane 5: PC-12, (Cell Lysate), (Prod No. ADI-LYC-PC100), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate) (Prod No. ADI-LYC-HL100).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10µg/ml.