NB120-2788

Gong Z, Tasset I, Diaz A, Anguiano J, Tas E, Cui L, Kuliawat R, Liu H, Kühn B, Cuervo AM, Muzumdar R
The Journal of cell biology 2018 Feb 5;217(2):635-647

Lysosomal dysfunction and early glial activation are involved in the pathogenesis of spinocerebellar ataxia type 21 caused by mutant transmembrane protein 240.

Seki T, Sato M, Kibe Y, Ohta T, Oshima M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H
Neurobiology of disease 2018 Dec;120:34-50

Early involvement of lysosome dysfunction in the degeneration of cerebral cortical neurons caused by the lipid peroxidation product 4-hydroxynonenal.

Zhang S, Eitan E, Mattson MP
Journal of neurochemistry 2017 Mar;140(6):941-954

Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle.

Ganesan S, Reynolds C, Hollinger K, Pearce SC, Gabler NK, Baumgard LH, Rhoads RP, Selsby JT
American journal of physiology. Regulatory, integrative and comparative physiology 2016 Jun 1;310(11):R1288-96

α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.

Kett LR, Stiller B, Bernath MM, Tasset I, Blesa J, Jackson-Lewis V, Chan RB, Zhou B, Di Paolo G, Przedborski S, Cuervo AM, Dauer WT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 8;35(14):5724-42

Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.

Park C, Suh Y, Cuervo AM
Nature communications 2015 Apr 16;6:6823

STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy.

Ferreira JV, Fôfo H, Bejarano E, Bento CF, Ramalho JS, Girão H, Pereira P
Autophagy 2013 Sep;9(9):1349-66

Interplay of LRRK2 with chaperone-mediated autophagy.

Orenstein SJ, Kuo SH, Tasset I, Arias E, Koga H, Fernandez-Carasa I, Cortes E, Honig LS, Dauer W, Consiglio A, Raya A, Sulzer D, Cuervo AM
Nature neuroscience 2013 Apr;16(4):394-406

Chaperone-mediated autophagy targets hypoxia-inducible factor-1α (HIF-1α) for lysosomal degradation.

Hubbi ME, Hu H, Kshitiz, Ahmed I, Levchenko A, Semenza GL
The Journal of biological chemistry 2013 Apr 12;288(15):10703-14

Altered lipid content inhibits autophagic vesicular fusion.

Koga H, Kaushik S, Cuervo AM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Aug;24(8):3052-65

Heat shock cognate protein 70 is involved in rotavirus cell entry.

Guerrero CA, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, Romero P, Méndez E, López S, Arias CF
Journal of virology 2002 Apr;76(8):4096-102

Platelet adhesion to collagen under flow causes dissociation of a phosphoprotein complex of heat-shock proteins and protein phosphatase 1.

Polanowska-Grabowska R, Simon CG Jr, Falchetto R, Shabanowitz J, Hunt DF, Gear AR
Blood 1997 Aug 15;90(4):1516-26

Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein.

Terlecky SR, Chiang HL, Olson TS, Dice JF
The Journal of biological chemistry 1992 May 5;267(13):9202-9

Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells.

Maekawa M, O'Brien DA, Allen RL, Eddy EM
Biology of reproduction 1989 Apr;40(4):843-52

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: Hsp70/ Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug whole cell lysate with 2ul of HSC70 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSC70 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with goat anti-mouse IgM-HRP secondary antibody for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Prostate carcinoma tissue.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Testis tissue.