MA5-17091

antibody from Invitrogen Antibodies

Targeting: HSP90AA1 FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17091

Provider

Invitrogen Antibodies

Product name

HSP90 alpha Monoclonal Antibody (5G5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17091 targets HSP90AA1 in FACS, IHC and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.

Antibody clone number

5G5

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HSP90/Heat Shock Protein 90 alpha using a HSP90/Heat Shock Protein 90 alpha monoclonal antibody (Product # MA5-17091) against a human HSP90AA1 (AA: 275-484) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK293T (Lane 1), HeLa (Lane 2), K-562 (Lane 3) and NIH/3T3 (Lane 4). The blot was probed with Anti-HSP90 alpha Monoclonal Antibody (5G5) (Product # MA5-17091, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 80 kDa band corresponding to HSP90 was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HSP90/Heat Shock Protein 90 alpha using a HSP90/Heat Shock Protein 90 alpha monoclonal antibody (Product # MA5-17091) against a human HSP90AA1 (AA: 275-484) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HSP90/Heat Shock Protein 90 alpha using HSP90/Heat Shock Protein 90 alpha monoclonal antibody (Product # MA5-17091) in NIH3T3 (1), HeLa (2), HCT116 (3), HL-60 (4) and C0S7 (5) cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using HSP90/Heat Shock Protein 90 alpha monoclonal antibody (Product # MA5-17091) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP90/Heat Shock Protein 90 alpha monoclonal antibody (Product # MA5-17091) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using HSP90/Heat Shock Protein 90 alpha monoclonal antibody (Product # MA5-17091) (green) and negative control (red).