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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX109753 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109753, RRID:AB_11178231
- Product name
- Hsp90 alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references LINC00261 Is an Epigenetically Regulated Tumor Suppressor Essential for Activation of the DNA Damage Response.
HSP-enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells.
Tauopathy-associated PERK alleles are functional hypomorphs that increase neuronal vulnerability to ER stress.
The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.
Achromatopsia mutations target sequential steps of ATF6 activation.
The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
TC-N19, a novel dual inhibitor of EGFR and cMET, efficiently overcomes EGFR-TKI resistance in non-small-cell lung cancer cells.
Expression patterns and structural modelling of Hsp70 and Hsp90 in a fish-borne zoonotic nematode Anisakis pegreffii.
Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence.
Effect of different temperatures on the expression of the newly characterized heat shock protein 90 (Hsp90) in L3 of Anisakis spp. isolated from Scomber australasicus.
Translational and posttranslational regulation of XIAP by eIF2α and ATF4 promotes ER stress-induced cell death during the unfolded protein response.
Andrographolide downregulates the v-Src and Bcr-Abl oncoproteins and induces Hsp90 cleavage in the ROS-dependent suppression of cancer malignancy.
The induction of heme oxygenase-1 suppresses heat shock protein 90 and the proliferation of human breast cancer cells through its byproduct carbon monoxide.
Shahabi S, Kumaran V, Castillo J, Cong Z, Nandagopal G, Mullen DJ, Alvarado A, Correa MR, Saizan A, Goel R, Bhat A, Lynch SK, Zhou B, Borok Z, Marconett CN
Cancer research 2019 Jun 15;79(12):3050-3062
Cancer research 2019 Jun 15;79(12):3050-3062
HSP-enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells.
Ono K, Eguchi T, Sogawa C, Calderwood SK, Futagawa J, Kasai T, Seno M, Okamoto K, Sasaki A, Kozaki KI
Journal of cellular biochemistry 2018 Sep;119(9):7350-7362
Journal of cellular biochemistry 2018 Sep;119(9):7350-7362
Tauopathy-associated PERK alleles are functional hypomorphs that increase neuronal vulnerability to ER stress.
Yuan SH, Hiramatsu N, Liu Q, Sun XV, Lenh D, Chan P, Chiang K, Koo EH, Kao AW, Litvan I, Lin JH
Human molecular genetics 2018 Nov 15;27(22):3951-3963
Human molecular genetics 2018 Nov 15;27(22):3951-3963
The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
Luu BE, Wijenayake S, Malik AI, Storey KB
Cell stress & chaperones 2018 Jan;23(1):45-53
Cell stress & chaperones 2018 Jan;23(1):45-53
Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.
Fan CS, Chen WS, Chen LL, Chen CC, Hsu YT, Chua KV, Wang HD, Huang TS
Oncotarget 2018 Jan 12;9(4):4998-5015
Oncotarget 2018 Jan 12;9(4):4998-5015
Achromatopsia mutations target sequential steps of ATF6 activation.
Chiang WC, Chan P, Wissinger B, Vincent A, Skorczyk-Werner A, Krawczyński MR, Kaufman RJ, Tsang SH, Héon E, Kohl S, Lin JH
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 10;114(2):400-405
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 10;114(2):400-405
The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
Chen X, Zhao X, Cai H, Sun H, Hu Y, Huang X, Kong W, Kong W
Redox biology 2017 Aug;12:987-1003
Redox biology 2017 Aug;12:987-1003
TC-N19, a novel dual inhibitor of EGFR and cMET, efficiently overcomes EGFR-TKI resistance in non-small-cell lung cancer cells.
Wu DW, Chen TC, Huang HS, Lee H
Cell death & disease 2016 Jun 30;7(6):e2290
Cell death & disease 2016 Jun 30;7(6):e2290
Expression patterns and structural modelling of Hsp70 and Hsp90 in a fish-borne zoonotic nematode Anisakis pegreffii.
Chen HY, Cheng YS, Shih HH
Veterinary parasitology 2015 Sep 15;212(3-4):281-91
Veterinary parasitology 2015 Sep 15;212(3-4):281-91
Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence.
Succoio M, Comegna M, D'Ambrosio C, Scaloni A, Cimino F, Faraonio R
Journal of proteomics 2015 Oct 14;128:18-29
Journal of proteomics 2015 Oct 14;128:18-29
Effect of different temperatures on the expression of the newly characterized heat shock protein 90 (Hsp90) in L3 of Anisakis spp. isolated from Scomber australasicus.
Chen HY, Cheng YS, Grabner DS, Chang SH, Shih HH
Veterinary parasitology 2014 Oct 15;205(3-4):540-50
Veterinary parasitology 2014 Oct 15;205(3-4):540-50
Translational and posttranslational regulation of XIAP by eIF2α and ATF4 promotes ER stress-induced cell death during the unfolded protein response.
Hiramatsu N, Messah C, Han J, LaVail MM, Kaufman RJ, Lin JH
Molecular biology of the cell 2014 May;25(9):1411-20
Molecular biology of the cell 2014 May;25(9):1411-20
Andrographolide downregulates the v-Src and Bcr-Abl oncoproteins and induces Hsp90 cleavage in the ROS-dependent suppression of cancer malignancy.
Liu SH, Lin CH, Liang FP, Chen PF, Kuo CD, Alam MM, Maiti B, Hung SK, Chi CW, Sun CM, Fu SL
Biochemical pharmacology 2014 Jan 15;87(2):229-42
Biochemical pharmacology 2014 Jan 15;87(2):229-42
The induction of heme oxygenase-1 suppresses heat shock protein 90 and the proliferation of human breast cancer cells through its byproduct carbon monoxide.
Lee WY, Chen YC, Shih CM, Lin CM, Cheng CH, Chen KC, Lin CW
Toxicology and applied pharmacology 2014 Jan 1;274(1):55-62
Toxicology and applied pharmacology 2014 Jan 1;274(1):55-62
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX109753 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Hsp90 alpha(GTX109753) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10£gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 £gg of Hsp90 alpha antibody (GTX109753).Western blot analysis was performed using Hsp90 alpha antibody (GTX109753).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Hsp90 alpha antibody (GTX109753) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody (GTX109753) diluted at 1:500.
