NB110-96872
antibody from Novus Biologicals
Targeting: HSP90AA1
FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB110-96872 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-96872, RRID:AB_1260801
- Product name
- Mouse Monoclonal HSP90 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes 90kDa. Can isolate complexes of HSP90, Src kinase and cec37.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens.
Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress.
Wang SH, Cheng CY, Chen CJ, Chan HL, Chen HH, Tang PC, Chen CF, Lee YP, Huang SY
Animal biotechnology 2019 Apr;30(2):129-145
Animal biotechnology 2019 Apr;30(2):129-145
Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress.
Wang SH, Cheng CY, Chen CJ, Chen HH, Tang PC, Chen CF, Lee YP, Huang SY
Theriogenology 2014 Jul 1;82(1):80-94
Theriogenology 2014 Jul 1;82(1):80-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSP90 Antibody (D7alpha) [NB110-96872] - analysis of Rat cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7Alpha . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP90 Antibody (D7alpha) [NB110-96872] - analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP90 Antibody (D7alpha) [NB110-96872] - analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.