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LearnPAB10219
antibody from Abnova Corporation
Targeting: HSP90AA1
FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10219 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10219, RRID:AB_1674717
- Product name
- HSP90AA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HSP90AA1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An acetylation site in the middle domain of Hsp90 regulates chaperone function.
HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor.
Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.
Scroggins BT, Robzyk K, Wang D, Marcu MG, Tsutsumi S, Beebe K, Cotter RJ, Felts S, Toft D, Karnitz L, Rosen N, Neckers L
Molecular cell 2007 Jan 12;25(1):151-9
Molecular cell 2007 Jan 12;25(1):151-9
HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor.
Kovacs JJ, Murphy PJ, Gaillard S, Zhao X, Wu JT, Nicchitta CV, Yoshida M, Toft DO, Pratt WB, Yao TP
Molecular cell 2005 May 27;18(5):601-7
Molecular cell 2005 May 27;18(5):601-7
Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.
Fuino L, Bali P, Wittmann S, Donapaty S, Guo F, Yamaguchi H, Wang HG, Atadja P, Bhalla K
Molecular cancer therapeutics 2003 Oct;2(10):971-84
Molecular cancer therapeutics 2003 Oct;2(10):971-84
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10219) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in an SK-BR-3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor).Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA).Immuno-precipitation with HSP90AA1 polyclonal antibody was performed prior to immunoblotting with anti-HSP90AA1 acetyl K294.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of HSP90AA1 polyclonal antibody (Cat # PAB10219) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
