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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- TA500883 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500883, RRID:AB_11141759
- Product name
- Anti-HSP90AA1 (Hsp90 alpha) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
- Antibody type
- Monoclonal
- Description
- Anti-HSP90AA1 (Hsp90 alpha) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSP90AA1
- Isotype
- IgG
- Antibody clone number
- OTI3B5
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Helicobacter pylori Affects the Antigen Presentation Activity of Macrophages Modulating the Expression of the Immune Receptor CD300E through miR-4270.
Pagliari M, Munari F, Toffoletto M, Lonardi S, Chemello F, Codolo G, Millino C, Della Bella C, Pacchioni B, Vermi W, Fassan M, de Bernard M, Cagnin S
Frontiers in immunology 2017;8:1288
Frontiers in immunology 2017;8:1288
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSP90AA1 (RC212496, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSP90AA1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-HSP90AA1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSP90AA1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500883, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HSP90AA1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500883, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-HSP90AA1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500883, Dilution 1:50)
- Validation comment
- IHC
