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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunohistochemistry [3]
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- Product number
- MA5-24135 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calbindin D28K Monoclonal Antibody (401025)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human S100P. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 401025
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Hyper-diversity of CRH interneurons in mouse hippocampus.
Gunn BG, Sanchez GA, Lynch G, Baram TZ, Chen Y
Brain structure & function 2019 Mar;224(2):583-598
Brain structure & function 2019 Mar;224(2):583-598
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human kidney tissue and human brain (cerebellum) tissue. PVDF membrane was probed with 0.25 µg/mL of mouse Anti-human Calbindin D Monoclonal Antibody (Product # MA5-24135) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Calbindin D at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human kidney tissue and human brain (cerebellum) tissue. PVDF membrane was probed with 0.25 µg/mL of mouse Anti-human Calbindin D Monoclonal Antibody (Product # MA5-24135) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Calbindin D at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calbindin D28K in human kidney tissue and human brain (cerebellum) tissue. Samples were incubated in Calbindin D28K monoclonal antibody (Product # MA5-24135) using a dilution of 0.25 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Calbindin D at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calbindin D28K in 0.2 mg/mL lysates of human brain (cerebellum) tissue. Samples were incubated in Calbindin D28K monoclonal antibody (Product # MA5-24135) at a dilution of 10 µg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Calbindin D28K Polyclonal Antibody (Product # MA5-24135) and a 27 kDa band corresponding to Calbindin D28K was observed across tissues tested except Mouse and Rat Heart which are reported to be negative. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4), Mouse Heart (Lane 5) and Rat Heart (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calbindin D28K in perfusion fixed frozen sections of rat brain (hypothalamus). Samples were incubated in Calbindin D28K monoclonal antibody (Product # MA5-24135) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calbindin D28K in immersion fixed paraffin-embedded sections of human brain (cerebellum). Samples were incubated in Calbindin D28K monoclonal antibody (Product # MA5-24135) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calbindin D28K in perfusion fixed paraffin-embedded sections of rat brainstem (medulla). Samples were incubated in Calbindin D28K monoclonal antibody (Product # MA5-24135) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red) and counterstained with hematoxylin (blue).
