STJ72890

antibody from St John's Laboratory

Targeting: CALB1 CALB

Western blot

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: STJ72890 - Provider product page
Provider: St John's Laboratory
Product name: Anti-calbindin D28 antibody (Internal) (STJ72890)
Antibody type: Polyclonal
Description: Goat polyclonal antibody anti-calbindin D28 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Reactivity: Human, Rat, Bovine, Canine, Porcine
Host: Goat
Conjugate: Unconjugated
Antigen sequence: KTFVDQYGQRDDGK
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20 on receipt and minimise freeze-thaw cycles.
Handling: NA

CALB1 protein structure - STJ72890 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72890 (0. 03µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72890 (0. 1µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72890 (0. 1µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA