PA5-85669

antibody from Invitrogen Antibodies

Targeting: CALB1 CALB

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85669

Provider

Invitrogen Antibodies

Product name

Calbindin D28K Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution. Predicted reactivity: Mouse (97%), Rat (98%), Pig (98%), Rabbit (97%), Chicken (80%), Bovine (100%). Positive Control: mouse brain, rat brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.51 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.

Ranea-Robles P, Portman K, Bender A, Lee K, He JC, Mulholland DJ, Argmann C, Houten SM
Kidney360 2021 Sep;2(9):1441-1454

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Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1.

Zhang Y, Varela L, Szigeti-Buck K, Williams A, Stoiljkovic M, Šestan-Peša M, Henao-Mejia J, D'Acunzo P, Levy E, Flavell RA, Horvath TL, Kaczmarek LK
Nature communications 2021 Mar 19;12(1):1731

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Cellular, Molecular and Biochemical Impacts of Silver Nanoparticles on Rat Cerebellar Cortex.

Mohamed EM, Kattaia AAA, Abdul-Maksoud RS, Abd El-Baset SA
Cells 2020 Dec 22;10(1)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Calbindin D28K was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin D28K Polyclonal Antibody (Product # PA5 85669) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 9 The Kv3.3 G592R mutation promotes release of exosomes into the extracellular space. a Representative transmission electron microscopy photomicrographs of low density fraction (LDF) and intermediate-density fraction (IDF) extracellular vesicles (EVs) isolated from the cerebellum of a wild type mouse. Scale bar: 500 nm. Images are representative of ten images taken of each of the sets analyzed. b Representative Western blot analyses of LDF EVs (L) and IDF EVs (I) compared to the cerebellar homogenate (BH). c Nanotrack analysis (NTA) of the hydrodynamic diameter of the EVs found in LDFs and IDFs. The curve is normalized to the mode of each distribution (five independent isolations. d Quantification of particles in LDFs and IDFs by NTA that are found within the size bins shown in the graph. Data are plotted as percentage of total number of EVs. Five independent isolations. e Representative Western blot analyses of EVs isolated from cerebella of wild type and G592R Kv3.3 mutant mice. LDFs (L) and IDFs (I) were isolated from cerebella of two mice of the same genotype and these were combined and tested for the markers shown. Two independent experiments are shown in the blot. f - h quantification of data in e . The densitometric quantification was performed on three independent experiments, representative of six mice per genotype. BCA protein assay ( i ) and total particle count as estimated by NTA ( j ) of LDFs and IDFs in wild type and G592R Kv3.3 mice. Values were normalize