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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [13]
- Other assay [1]
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- Product number
- 14479-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calbindin-D28k Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KYDTDHSGF IETEELKNFL KDLLEKANKT VDDTKLAEYT DLMLKLFDSN NDGKLELTEM ARLLPVQENF LLKFQGIKMC GKEFNKAFEL YDQDGNGYID ENELDALLKD LCEKNKQDLD INNITTYKKN IMALSDGGKL YRTDLALILC AGDN (109-262 aa encoded by B C006478)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 14479-1-AP (CALB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14479-1-AP ( Calbindin-D28k antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 14479-1-AP (Calbindin-D28k antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IHC result of Calbindin antibody (14479-1-AP, 1:5000 in 1% BSA 0.3%Tx-100 Tris buffer incubated at 4°C overnight) with freshly formalin fixed human hippocampus (floating section). Secondary antibody biotinylated anti-rabbit lgG 1:200 in 1% BSA 0.3%Tx-100 Tris buffer for 2 hours at room temperature.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (Product # 14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Calbindin-D28k (IP:14479-1-AP, 4ug; Detection:14479-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
