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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32284 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Calbindin D28K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast ductal carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C, do not freeze
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Calbindin D28 using anti-Calbindin D28 Polyclonal Antibody (Product # PA5-32284) in Cerebellum Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:400.
