MA1-22165

antibody from Invitrogen Antibodies

Targeting: SELE CD62E, ELAM, ELAM1, ESEL

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-22165

Provider

Invitrogen Antibodies

Product name

E-selectin Monoclonal Antibody (1.2B6)

Antibody type

Monoclonal

Antigen

Other

Description

Not suitable for immunohistochemistry (paraffin). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

1.2B6

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray.

Bæk R, Søndergaard EK, Varming K, Jørgensen MM
Journal of immunological methods 2016 Nov;438:11-20

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Potentials and capabilities of the Extracellular Vesicle (EV) Array.

Jørgensen MM, Bæk R, Varming K
Journal of extracellular vesicles 2015;4:26048

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Potentials and capabilities of the Extracellular Vesicle (EV) Array.

Jørgensen MM, Bæk R, Varming K
Journal of extracellular vesicles 2015;4:26048

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Novel anti-inflammatory properties of the angiogenesis inhibitor vasostatin.

Huegel R, Velasco P, De la Luz Sierra M, Christophers E, Schröder JM, Schwarz T, Tosato G, Lange-Asschenfeldt B
The Journal of investigative dermatology 2007 Jan;127(1):65-74

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of E-selectin was performed using 70% confluent log phase HUVEC cells treated with 100 U/mL TNF-alpha for 3 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with E-selectin Monoclonal Antibody (1.2B6) (Product # MA1-22165) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membrane localization. Panel e represents untreated cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of Collagen I was performed in thrombin activated human peripheral blood platelets using Collagen I Polyclonal Antibody, Biotin (Product # PA1-28530).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL