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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- BBA26-200 - Provider product page
- Provider
- R&D Systems
- Product name
- Human E-Selectin/CD62E Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody binds to CHO cells transfected with human E-Selectin. It does not bind to COS cells transfected with human PECAM-1, P-Selectin, L-Selectin, VCAM-1, or ICAM-1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- BBIG-E1
- Vial size
- 200 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium.
A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells.
Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
Lam FW, Da Q, Guillory B, Cruz MA
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1718-1726
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1718-1726
A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells.
Pachón-Peña G, Donnelly C, Ruiz-Cañada C, Katz A, Fernández-Veledo S, Vendrell J, Sackstein R
Stem cells (Dayton, Ohio) 2017 Apr;35(4):1080-1092
Stem cells (Dayton, Ohio) 2017 Apr;35(4):1080-1092
Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin BV, Zarbock A, Gerke V, Rescher U
Nature communications 2014 Apr 28;5:3738
Nature communications 2014 Apr 28;5:3738
Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
Pezeshkian B, Donnelly C, Tamburo K, Geddes T, Madlambayan GJ
PloS one 2013;8(4):e60823
PloS one 2013;8(4):e60823
The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
The Journal of biological chemistry 2006 Jul 21;281(29):20148-59
The Journal of biological chemistry 2006 Jul 21;281(29):20148-59
Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
Lawson C, Ainsworth M, Yacoub M, Rose M
Journal of immunology (Baltimore, Md. : 1950) 1999 Mar 1;162(5):2990-6
Journal of immunology (Baltimore, Md. : 1950) 1999 Mar 1;162(5):2990-6
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- E-Selectin/CD62E in HUVECs. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
