BMS1014

antibody from Invitrogen Antibodies

Targeting: SELE CD62E, ELAM, ELAM1, ESEL

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

BMS1014

Provider

Invitrogen Antibodies

Product name

CD62E (E-selectin) Monoclonal Antibody (CL-37), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: E-Selectin (CL-37) is a mouse monoclonal antibody raised against E-Selectinof human origin. Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules. Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells. Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail. Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation. Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation. Applications Tested: ELISA, Immunohistochemistry (Frozen). Source: Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. accession number NP_000580. Bioactivity: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg. Endotoxin: Less than 0.005 ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE. Molecular Weight: 17.2 kDa (apparent 23 and 24 kDa).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CL-37

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C