12-0627-42

antibody from Invitrogen Antibodies
Targeting: SELE CD62E, ELAM, ELAM1, ESEL
Provider product page for 12-0627-42

Antibody data

Product number
12-0627-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium. The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells. Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P2H3 antibody has been pre-titrated and tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
P2H3
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
SELE protein structure - 12-0627-42 shown in red.