Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-33306PE - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM/CD66 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody recognizes proteins of 80-200kDa, identified as different members of CEACAM family. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEACAM is not found in benign glands, stroma, or malignant prostatic cells
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: CEACAM/CD66 Antibody (COL-1) [PE] [NBP2-33306PE] - An intracellular stain was performed on A549 cells with CEACAM/CD66 Antibody (COL-1) NBP2-33306PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.