Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-36277 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM1/CD66a Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Anti-human CEACAM-1 (B3) interacts solely with human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in human or other species, Clone B3 binds to the A1-B-Domain.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEACAM1/CD66a Antibody (B3-17) [NBP2-36277] - Human CEACAM-1 lysate. Detection utilizing 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CEACAM1/CD66a Antibody (B3-17) [NBP2-36277] - Solid phase was coated with 3 Ug/ml anti CEA (Dako) binding human CEACAM-1 and CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody B3-17 (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (B3-17) [NBP2-36277] - CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using mAb B3 followed by staining with antimouse HRP-DAB and counterstaining with hematoxylin. The labeling showed week staining of CEACAM1 by B3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CEACAM1/CD66a Antibody (B3-17) [NBP2-36277] - Stably transfected HeLa cells: 250.000 Helahuman CEACAM1 cells; 10 ug/ml of primary B3-17. Binding was detected with a FITC-conjugated secondary antibody.