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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Flow cytometry [1]
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- Product number
- AM26011PU-N - Provider product page
- Provider
- OriGene
- Product name
- CEACAM1 mouse monoclonal antibody, clone C5-1X, Purified
- Antibody type
- Monoclonal
- Description
- CEACAM1 mouse monoclonal antibody, clone C5-1X, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM1
- Isotype
- IgG
- Antibody clone number
- C5-1X
- Vial size
- 100 µg
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot: Human CEACAM-1 lysate Detection utilysing 10 ug/ml.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sandwich ELISA: Solid phase was coated with 3 ug/ml anti CEA binding human CEACAM1- CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody mAb C5-1X (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometry: 10 ug/ml of primary mAb C5-1X; 250.000 Hela-human CEACAM1 cells.
- Validation comment
- FC
