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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- DM1201 - Provider product page
- Provider
- OriGene
- Product name
- CEACAM1 mouse monoclonal antibody, clone 8G5, Purified
- Antibody type
- Monoclonal
- Description
- CEACAM1 mouse monoclonal antibody, clone 8G5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM1
- Isotype
- IgG
- Antibody clone number
- 8G5
- Vial size
- 100 µg
- Concentration
- 2.0 mg/ml
Submitted references The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma.
Kammerer R, Riesenberg R, Weiler C, Lohrmann J, Schleypen J, Zimmermann W
The Journal of pathology 2004 Nov;204(3):258-67
The Journal of pathology 2004 Nov;204(3):258-67
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining on cryosections of colon tissue using 8G5 . PBS containing 2.5% horse serum served as negative control (A). Detection of 8G5 occurred with a bioti-nylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminoben-zidine was used as substrate. Nuclei were stained with hematoxilin.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,5,6,7,8,19,20 or 21. Recognition of CEACAM3 and 4 was tested on stably trans-fected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1,3,4: D14HD11; CEACAM5: 26/3/13; CEACAM6: 9A6; CEACAM7: BAC2; CEACAM8: 2H6; CEACAM19-21: anti-myc; green curves). An irrelevant monoclonal antibody served as a nega-tive control (black curves). For specificity testing, protein G-purified 8G5 was tested on all CEACAM transfectants. A posi-tive signal was only obtained with CEACAM1-expressing cells (red curves).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using 8G5 . BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of 8G5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM1 transfected cells.
- Validation comment
- FC
