MA5-23908

antibody from Invitrogen Antibodies

Targeting: CEACAM1 BGP, BGP1, CD66a

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23908

Provider

Invitrogen Antibodies

Product name

CEACAM1 Monoclonal Antibody (283324)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1 was observed. In sandwich ELISAs, no cross-reactivity with rhCEACAM-3, rhCEACAM-5, or rhCEACAM-6 was observed.

Antibody clone number

283324

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CEACAM1 in human liver tissue and HepG2 human hepatocellular carcinoma cell line. Samples were incubated in CEACAM1 monoclonal antibody (Product # MA5-23908) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. Specific bands were detected for CEACAM‚1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CEACAM1 in immersion fixed paraffin-embedded sections of human colon. Samples were incubated in CEACAM1 monoclonal antibody (Product # MA5-23908) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to stromal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CEACAM1 in immersion fixed paraffin-embedded sections of human colon cancer tissue. Samples were incubated in CEACAM1 monoclonal antibody (Product # MA5-23908) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).