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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A700-032-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CEACAM1 Recombinant Rabbit Monoclonal Antibody (BLR032F)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR032F
- Vial size
- 10 µL
- Concentration
- 50 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CEACAM1/5 by western blot. Samples: Whole cell lysate (5 µg) from UACC-257, SK-MEL-28, Hep-G2, SUP-M2, HeLa, HEK293T, Jurkat, RAW 264.7, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody [BLR032F] (Product # A700-032 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin (Product # A300-491A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CEACAM1 by WB of immunoprecipitates from UACC-257 lysate. Antibodies: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (A700-032) and rabbit anti-CEACAM1 recombinant monoclonal [294-4G7]. Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CEACAM1 by WB from UACC-257, SK-MEL28, HepG2, SUP-M2, HeLa, 293T, Jurkat, RAW 264.7, and TCMK-1 lysate. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (A700-032). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-Actin (A300-491A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CEACAM1 in FFPE HT29 cells by ICC.Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (A700-032).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CEACAM1/5 by immunohistochemistry.Sample: FFPE section of colon carcinoma.Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody [BLR032F] (Product # A700-032 lot 1) used at 1:500.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CEACAM1/5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP; 20% of IP loaded) from UACC-257 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody [BLR032F] (Product # A700-032 lot 1) used for IP at 20 µL per reaction. CEACAM1/5 was also immunoprecipitated by rabbit anti-CEACAM1/5 recombinant monoclonal antibody [294-4G7]. For blotting immunoprecipitated CEACAM1/5 (Product # A700-032) was used at 1:1000. Chemiluminescence with an exposure time of 1 second.
