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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- ALX-805-076-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-805-076-C100, RRID:AB_2051113
- Product name
- CEACAM1 (human) monoclonal antibody (GM8G5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Protein G-affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GM8G5
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
Submitted references Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.
CD66b regulates adhesion and activation of human eosinophils.
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
CD66b regulates adhesion and activation of human eosinophils.
Yoon J, Terada A, Kita H
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8454-62
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8454-62
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistological staining of colon tissue with MAb to CEACAM1 (GM8G5) (Prod. No. ALX-805-076). Cryosections of colon tissue were stained with 10 µg/ml GM8G5 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representa-tive sections of analysed tissues. Detection of GM8G5 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate. Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Specificity testing of GM8G5. BOSC cells were transiently transfected with an expression vector containing the cDNA of CEACAM1. Expression of the constructs was tested with MAb to CEACAm1 (GM8G5) (Prod.No. ALX-805-076) (B, purple curves); an irrelevant monoclonal antibody served as negative control (red curve). For specificity testing (A), GM8G5 hybridoma supernatant was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM1 transfected cells (purple curve).
