AM26011PU-N

antibody from Acris Antibodies GmbH

Targeting: CEACAM1 BGP, BGP1, CD66a

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM26011PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM26011PU-N, RRID:AB_10845414

Product name

anti CD66a / CEACAM1

Antibody type

Monoclonal

Antigen

Recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

C5-1X

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot: Human CEACAM-1 lysate Detection utilysing 10 μg/ml.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Sandwich ELISA: Solid phase was coated with 3 μg/ml anti CEA binding human CEACAM1- CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody mAb C5-1X (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometry: 10 μg/ml of primary mAb C5-1X; 250.000 Hela-human CEACAM1 cells.