DM1201

antibody from Acris Antibodies GmbH

Targeting: CEACAM1 BGP, BGP1, CD66a

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1201

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM1201, RRID:AB_977063

Product name

anti CD66a / CEACAM1

Antibody type

Monoclonal

Antigen

Genetic immunisation with cDNA encoding the extracellular region of Human CEACAM1-A2.Based on recognition of the complete native protein expressed on transfected mammalian cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8G5

Vial size

0.1 mg

Concentration

1.0 mg/ml

References

Submitted references

The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays.

Yue T, Goldstein IJ, Hollingsworth MA, Kaul K, Brand RE, Haab BB
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1697-707

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Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.

Sémiramoth N, Gleizes A, Turbica I, Sandré C, Gorges R, Kansau I, Servin A, Chollet-Martin S
Journal of leukocyte biology 2009 Feb;85(2):310-21

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Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes.

Béaslas O, Torreilles F, Casellas P, Simon D, Fabre G, Lacasa M, Delers F, Chambaz J, Rousset M, Carrière V
American journal of physiology. Gastrointestinal and liver physiology 2008 Nov;295(5):G942-52

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Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.

Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55

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The CEACAM1-mediated apoptosis pathway is activated by CEA and triggers dual cleavage of CEACAM1.

Nittka S, Böhm C, Zentgraf H, Neumaier M
Oncogene 2008 Jun 12;27(26):3721-8

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CD66b regulates adhesion and activation of human eosinophils.

Yoon J, Terada A, Kita H
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8454-62

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Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner.

Avadhanula V, Rodriguez CA, Devincenzo JP, Wang Y, Webby RJ, Ulett GC, Adderson EE
Journal of virology 2006 Feb;80(4):1629-36

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The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma.

Kammerer R, Riesenberg R, Weiler C, Lohrmann J, Schleypen J, Zimmermann W
The Journal of pathology 2004 Nov;204(3):258-67

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical staining on cryosections of colon tissue using 8G5 (DM1201). PBS containing 2.5% horse serum served as negative control (A). Detection of 8G5 occurred with a bioti-nylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminoben-zidine was used as substrate. Nuclei were stained with hematoxilin.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical staining on cryosections of colon tissue using 8G5 (DM1201). PBS containing 2.5% horse serum served as negative control (A). Detection of 8G5 occurred with a bioti-nylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminoben-zidine was used as substrate. Nuclei were stained with hematoxilin.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis of BOSC23 cells using 8G5 (DM1201). BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of 8G5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM1 transfected cells.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,5,6,7,8,19,20 or 21. Recognition of CEACAM3 and 4 was tested on stably trans-fected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1,3,4: D14HD11; CEACAM5: 26/3/13; CEACAM6: 9A6; CEACAM7: BAC2; CEACAM8: 2H6; CEACAM19-21: anti-myc; green curves). An irrelevant monoclonal antibody served as a nega-tive control (black curves). For specificity testing, protein G-purified 8G5 was tested on all CEACAM transfectants. A posi-tive signal was only obtained with CEACAM1-expressing cells (red curves).