MAB8126

antibody from R&D Systems

Targeting: C9

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8126

Provider

R&D Systems

Product name

Human Complement Component C9 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Complement Component C9 in ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P02748


Isotype

IgG

Antibody clone number

887025

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Complement Component C9 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Complement Component C9 by Simple Western^TM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Complement Component C9 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.