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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- PA5-29093 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Complement C9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: THP-1, BCL-1, human plasma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.62 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references GDF15 Suppresses Lymphoproliferation and Humoral Autoimmunity in a Murine Model of Systemic Lupus Erythematosus.
Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.
Lorenz G, Ribeiro A, von Rauchhaupt E, Würf V, Schmaderer C, Cohen CD, Vohra T, Anders HJ, Lindenmeyer M, Lech M
Journal of innate immunity 2022;14(6):673-689
Journal of innate immunity 2022;14(6):673-689
Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.
Lee JH, Jung JH, Kim J, Baek WK, Rhee J, Kim TH, Kim SH, Kim KP, Son CN, Kim JS
Clinical proteomics 2020;17:20
Clinical proteomics 2020;17:20
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C9 using 30 µg of BCL-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a C9 polyclonal antibody (Product # PA5-29093) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Complement C9 was performed by separating 50 µg of mouse plasma by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Complement C9 Polyclonal Antibody (Product # PA5-29093) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Complement C9 was performed by separating 30 µg of Human plasma by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Complement C9 Polyclonal Antibody (Product # PA5-29093) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Complement C9 was performed by separating 50 µg of rat plasma by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Complement C9 Polyclonal Antibody (Product # PA5-29093) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Complement C9 Polyclonal Antibody (Product # PA5-29093). Sample (30 µg of whole cell lysate). Lane A: THP-1. 7.5% SDS PAGE. Complement C9 Polyclonal Antibody (Product # PA5-29093) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Complement C9 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Complement C9 Polyclonal Antibody (Product # PA5 29093) diluted at 1:500. Blue: Hoechst 33342 staining.
