Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- NBP1-48348AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin, pan Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This Mouse monoclonal Cytokeratin (Pan-reactive) antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification.
Ijsselsteijn ME, Brouwer TP, Abdulrahman Z, Reidy E, Ramalheiro A, Heeren AM, Vahrmeijer A, Jordanova ES, de Miranda NF
The journal of pathology. Clinical research 2019 Jan;5(1):3-11
The journal of pathology. Clinical research 2019 Jan;5(1):3-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: pan Cytokeratin Antibody (C-11) [Alexa Fluor 647] [NBP1-48348AF647] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.