H00003851-M01

antibody from Abnova Corporation

Targeting: KRT4 CK4, CYK4, K4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003851-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003851-M01, RRID:AB_530105

Product name

KRT4 monoclonal antibody (M01), clone 5H5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant KRT4.

Antigen sequence

SSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELK
TMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVD
AAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQ
TH


Isotype

IgG

Antibody clone number

5H5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

KRT4 monoclonal antibody (M01), clone 5H5 Western Blot analysis of KRT4 expression in A-431 ( Cat # L015V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged KRT4 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to KRT4 on A-431 cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to KRT4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol