14-9000-80

antibody from Invitrogen Antibodies
Targeting: KRT4 CK4, CYK4, K4
Provider product page for 14-9000-80
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
14-9000-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-Cytokeratin Pan Type II Monoclonal Antibody (AE3), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody AE3 recognizes many members of the basic (Type II) cytokeratin family. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm. The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins (also known as cytokeratin 1, 2, 3, 4, 5, 6, 7 and 8) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken. Applications Reported: This AE3 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This AE3 antibody has been by tested western blot on SDS-reduced lysates from MCF7 cells at less than or equal to 2 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Canine, Chicken/Avian, Rabbit
Host
Mouse
Isotype
IgG
Antibody clone number
AE3
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
KRT4 protein structure - 14-9000-80 shown in red.