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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29463 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 4 Polyclonal Antibody (Product # PA5-29463). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. Cytokeratin 4 Polyclonal Antibody (Product # PA5-29463) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytokeratin 4 Polyclonal Antibody detects KRT4 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: KRT4 protein stained by Cytokeratin 4 Polyclonal Antibody (Product # PA5-29463) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Cytokeratin 4 (Product # PA5-29463) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
