701288

antibody from Invitrogen Antibodies

Targeting: MMP10 STMY2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

701288

Provider

Invitrogen Antibodies

Product name

MMP10 Recombinant Rabbit Monoclonal Antibody (24 H24L21)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Antibody clone number

24 H24L21

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Rat pancreas (20 µg). Endogenous level of MMP10 was detected at ~ 54 kDa using MMP10 Recombinant Rabbit Monoclonal Antibody (Product # 701288) at 2 µg/mL. The blot was developed using chemiluminescence (ECL) method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP10 was performed by loading 20 µg of Raji (lane1), HeLa (lane2), HEK-293 (lane3), Jurkat (lane4) and U2OS (lane5) cell lysates using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. MMP10 was detected at ~54 kDa using MMP10 Recombinant Rabbit Monoclonal Antibody (Product # 701288) at 1-3 µg/mL dilution in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG-HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP-10 in rat pancreas lysate using a MMP-10 recombinant rabbit monoclonal antibody (Product # 701288) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~54kDa.