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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- 701288 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP10 Recombinant Rabbit Monoclonal Antibody (24 H24L21)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 24 H24L21
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Rat pancreas (20 µg). Endogenous level of MMP10 was detected at ~ 54 kDa using MMP10 Recombinant Rabbit Monoclonal Antibody (Product # 701288) at 2 µg/mL. The blot was developed using chemiluminescence (ECL) method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP10 was performed by loading 20 µg of Raji (lane1), HeLa (lane2), HEK-293 (lane3), Jurkat (lane4) and U2OS (lane5) cell lysates using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. MMP10 was detected at ~54 kDa using MMP10 Recombinant Rabbit Monoclonal Antibody (Product # 701288) at 1-3 µg/mL dilution in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG-HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP-10 in rat pancreas lysate using a MMP-10 recombinant rabbit monoclonal antibody (Product # 701288) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~54kDa.
