710312

antibody from Invitrogen Antibodies

Targeting: MMP10 STMY2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

710312

Provider

Invitrogen Antibodies

Product name

MMP10 Recombinant Polyclonal Antibody (24HCLC)

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

24HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP-10 in whole tissue extracts from rat pancreas using a MMP-10 Recombinant Rabbit Polyclonal Antibody (Product # 710312) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~54kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP10 was performed by loading 30 µg of Mouse Pancreas (lane1) and Rat Pancreas (lane2) tissue lysates using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. MMP10 was detected at ~30 kDa using MMP10 Recombinant Rabbit Polyclonal Antibody (Product # 710312) at 1-3 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG-HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).