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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- PA5-48065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLXNB3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Plexin B3 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL Sheep Anti-human Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48065) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, recombinant human Plexin B3 (1 ng) was included. A specific band for Plexin B3 was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLXNB3 in rat embryonic hippocampal neurons. Samples were incubated in PLXNB3 polyclonal antibody (Product # PA5-48065) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. For additional reference, Recombinant Human Plexin B3 (1 ng) was included. A specific band for Plexin B3 was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PLXNB3 in A172 human glioblastoma cell line. Samples were incubated in PLXNB3 polyclonal antibody (Product # PA5-48065) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.
