Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [5]
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- Product number
- LS-C94332 - Provider product page
- Provider
- LSBio
- Product name
- NOS1 / nNOS Antibody (C-Terminus) LS-C94332
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Rabbit
- Host
- Goat
- Isotype
- IgG
- Storage
- Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat antibody to NOS1. IHC on paraffin sections of mouse brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat antibody to NOS1. IHC on paraffin sections of human brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat antibody to NOS1. IHC on paraffin sections of mouse olfactory bulb tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Goat antibody to NOS1. IHC on paraffin sections of mouse brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Goat antibody to NOS1. IHC on paraffin sections of human brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.