LS-C94332

antibody from LSBio

Targeting: NOS1 nNOS, NOS

Western blot

Antibody data

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Validation data

Product number: LS-C94332 - Provider product page
Provider: LSBio
Product name: NOS1 / nNOS Antibody (C-Terminus) LS-C94332
Antibody type: Polyclonal
Description: Purified
Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit
Host: Goat
Isotype: IgG
Storage: Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.

NOS1 protein structure - LS-C94332 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of mouse brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of human brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of mouse olfactory bulb tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of mouse brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of human brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of mouse olfactory bulb tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of mouse brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Goat antibody to NOS1. IHC on paraffin sections of human brain tissue using Goat antibody to NOS1. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.