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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- OSN00004G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- nNOS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute lyophilized product with 1 mL distilled water.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Rabbit
- Host
- Goat
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Submitted references Unique Neural Circuit Connectivity of Mouse Proximal, Middle, and Distal Colon Defines Regional Colonic Motor Patterns.
Nestor-Kalinoski A, Smith-Edwards KM, Meerschaert K, Margiotta JF, Rajwa B, Davis BM, Howard MJ
Cellular and molecular gastroenterology and hepatology 2022;13(1):309-337.e3
Cellular and molecular gastroenterology and hepatology 2022;13(1):309-337.e3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-nNOS Polyclonal Antibody (Product # OSN00004G) and a 160kDa band corresponding to nNOS was observed in Mouse Skeletal Muscle but not in Mouse Heart (DOI: 10.1006/niox.2000.0300). An additional band at ~80kDa was observed in Mouse Heart. Whole cell extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1) and Mouse Heart (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (10µg/mL) and detected by chemiluminescence Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human brain tissue using nNOS Polyclonal Antibody (Product # OSN00004G). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:100, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of mouse brain tissue using nNOS Polyclonal Antibody (Product # OSN00004G). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:100, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of mouse olfactory bulb tissue using nNOS Polyclonal Antibody (Product # OSN00004G). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:100, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
