- 37-2800 - Invitrogen Antibodies NOS1 antibody

Submitted references In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.

Wind M, Tsakiridis A
Current protocols 2021 Sep;1(9):e244

Stenosis coexists with compromised α1-adrenergic contractions in the ascending aorta of a mouse model of Williams-Beuren syndrome.

Jiménez-Altayó F, Ortiz-Romero P, Puertas-Umbert L, Dantas AP, Pérez B, Vila E, D'Ocon P, Campuzano V
Scientific reports 2020 Jan 21;10(1):889

Preventive Effects of Different Fermentation Times of Shuidouchi on Diphenoxylate-Induced Constipation in Mice.

Chen L, Zhang J, Suo H, Wang W, Wang H, Zhang Y, Hu Q, Zhao X, Li J
Foods (Basel, Switzerland) 2019 Mar 1;8(3)

Neuromodulatory Action of Picomolar Extracellular Aβ42 Oligomers on Presynaptic and Postsynaptic Mechanisms Underlying Synaptic Function and Memory.

Gulisano W, Melone M, Ripoli C, Tropea MR, Li Puma DD, Giunta S, Cocco S, Marcotulli D, Origlia N, Palmeri A, Arancio O, Conti F, Grassi C, Puzzo D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Jul 24;39(30):5986-6000

The role of AMP-activated protein kinase in the expression of the dystrophin-associated protein complex in skeletal muscle.

Dial AG, Rooprai P, Lally JS, Bujak AL, Steinberg GR, Ljubicic V
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jun;32(6):2950-2965

NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels.

Bastian C, Zaleski J, Stahon K, Parr B, McCray A, Day J, Brunet S, Baltan S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 11;38(28):6247-6266

Increased expression of carbon monoxide-producing enzymes in the MPOA after sexual experience in male rats.

Robison CL, McHenry JA, Hull EM
Physiology & behavior 2017 Mar 15;171:149-157

Inhibition of NLRP3 Inflammasome Prevents LPS-Induced Inflammatory Hyperalgesia in Mice: Contribution of NF-κB, Caspase-1/11, ASC, NOX, and NOS Isoforms.

Dolunay A, Senol SP, Temiz-Resitoglu M, Guden DS, Sari AN, Sahan-Firat S, Tunctan B
Inflammation 2017 Apr;40(2):366-386

Intracallosal neuronal nitric oxide synthase neurons colocalize with neurokinin 1 substance P receptor in the rat.

Barbaresi P, Mensà E, Lariccia V, Desiato G, Fabri M, Gratteri S
The Journal of comparative neurology 2015 Mar 1;523(4):589-607

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Barbaresi P, Mensà E, Lariccia V, Pugnaloni A, Amoroso S, Fabri M
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Aguirre E, López-Bernardo E, Cadenas S
Mitochondrion 2012 Jan;12(1):126-31

Correlation between hippocampal levels of neural, epithelial and inducible NOS and spatial learning skills in rats.

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of rat brain tissue using nNOS Monoclonal Antibody, Mouse (clone 3G6B10) (Product # 37-2800).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of nNOS showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- nNOS Monoclonal Antibody (3G6B10) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of nNOS was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with nNOS Mouse Monoclonal Antibody (372800, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Na V 1.4 co-precipitates with members of the dystrophin associated protein complex (DAPC). Using an antibody generated against a peptide corresponding to the highly conserved III-IV linker region (pan Na V 1.x), sodium channels from NP40-solubilized control and CIM skeletal muscle membranes were immunoprecipitated. The immunoprecipitated (IP) control (Con) or CIM materials were resolved on SDS-PAGE gels and probed in western blots with the indicated antibodies. As a negative control, the IP was performed in the presence of blocking peptide (IP/pep), the same sequence used as the antigen; and as a positive control, starting membranes were used (Memb). ( A ) A full-panel western with the Na V 1.4-specific monoclonal antibody LD3 is shown on the left in comparison to a protein gel stained with SYPRO Ruby protein stain on the right. The denatured antibody heavy chain (Ab HC) and light chain (Ab LC) are present in the immunoprecipitated samples, as best seen in the SYPRO stained gel. A large number of proteins co-precipitate with the sodium channel (Na V 1.x), and many of these are shown to be specific since they are absent in the peptide control. ( B ) Using antibodies against plectin, dystrophin (dys), neuronal nitric oxide synthase (nNOS), syntrophin (syn), and beta-dystroglycan (beta-dys) confirms that many components of the DAPC are present in the control and CIM IPs. ( C ) Quantification of the data from panel B. For each antibody in each CoIP, the signal for the co

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 nNOS is expressed in the sarcolemma of control and CIM muscle. Cross-sections of medial gastrocnemius muscle were stained with the antibodies to nNOS as well as dystrophin to mark muscle surface membrane. In both control and CIM, nNOS is expressed in the surface membrane. The myofiber cross-sections are much smaller in CIM muscle, due to the previously reported muscle atrophy in the disease [ 10 ].

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Genic and protein expression in thoracic aortas from wild-type and CD mice. ( A ) qPCR mRNA expression of Limk1 , a gene contained in the Williams-Beuren syndrome critical region, and Adra1a (alpha1 A -adrenoceptors). Results are the mean +- SEM from wild-type (n = 4) and CD (n = 5) mice. * P < 0.05; *** P < 0.01 by Multiple t-test with Sidak-Bonferroni. ( B ) Western blot analysis for nNOS protein expression. Bar graphs show the results of densitometric analyses from pooled data for nNOS, iNOS, and eNOS protein expression. The molecular weight (kDa) of the protein is shown on the right side of the blot. Total protein was used as a loading control. Results are the mean +- SEM from wild-type (n = 5) and CD (n = 5) mice. * P < 0.05 by Mann-Whitney U test. ( C ) Representative photomicrographs and quantification of immunofluorescence (red) for nNOS, iNOS, and eNOS in confocal microscopic aortic sections. Scale bar, 20 mum. Results are the mean +- SEM from wild-type (n = 8) and CD (n = 8) mice. * P < 0.05 by Student's t-test.

37-2800

Antibody data