PA1-032

antibody from Invitrogen Antibodies

Targeting: NOS1 nNOS, NOS

Provider product page for PA1-032

Western blot

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [11]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Other assay [9]

Submit

Product number: PA1-032 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-nNOS (Ser1417) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-032 detects phosphorylated nNOS (Ser 1417) from rat and human samples.
Concentration: 1 mg/mL

NOS1 protein structure - PA1-032 shown in red.

Submitted references AT1R-AT2R-RXFP1 Functional Crosstalk in Myofibroblasts: Impact on the Therapeutic Targeting of Renal and Cardiac Fibrosis.

Chow BSM, Kocan M, Shen M, Wang Y, Han L, Chew JY, Wang C, Bosnyak S, Mirabito-Colafella KM, Barsha G, Wigg B, Johnstone EKM, Hossain MA, Pfleger KDG, Denton KM, Widdop RE, Summers RJ, Bathgate RAD, Hewitson TD, Samuel CS
Journal of the American Society of Nephrology : JASN 2019 Nov;30(11):2191-2207

Exercise training augments neuronal nitric oxide synthase dimerization in the paraventricular nucleus of rats with chronic heart failure.

Sharma NM, Liu X, Llewellyn TL, Katsurada K, Patel KP
Nitric oxide : biology and chemistry 2019 Jun 1;87:73-82

Serelaxin inhibits the profibrotic TGF-β1/IL-1β axis by targeting TLR-4 and the NLRP3 inflammasome in cardiac myofibroblasts.

Cáceres FT, Gaspari TA, Samuel CS, Pinar AA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Dec;33(12):14717-14733

A microRNA switch regulates the rise in hypothalamic GnRH production before puberty.

Messina A, Langlet F, Chachlaki K, Roa J, Rasika S, Jouy N, Gallet S, Gaytan F, Parkash J, Tena-Sempere M, Giacobini P, Prevot V
Nature neuroscience 2016 Jun;19(6):835-44

NO-dependent protective effect of VEGF against excitotoxicity on layer VI of the developing cerebral cortex.

El Ghazi F, Desfeux A, Brasse-Lagnel C, Roux C, Lesueur C, Mazur D, Remy-Jouet I, Richard V, Jégou S, Laudenbach V, Marret S, Bekri S, Prevot V, Gonzalez BJ
Neurobiology of disease 2012 Mar;45(3):871-86

Enhancement of rostral ventrolateral medulla neuronal nitric-oxide synthase-nitric-oxide signaling mediates the central cannabinoid receptor 1-evoked pressor response in conscious rats.

Ibrahim BM, Abdel-Rahman AA
The Journal of pharmacology and experimental therapeutics 2012 Jun;341(3):579-86

Kisspeptin-GPR54 signaling in mouse NO-synthesizing neurons participates in the hypothalamic control of ovulation.

Hanchate NK, Parkash J, Bellefontaine N, Mazur D, Colledge WH, d'Anglemont de Tassigny X, Prevot V
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jan 18;32(3):932-45

Differential modulation by vascular nitric oxide synthases of the ethanol-evoked hypotension and autonomic dysfunction in female rats.

El-Mas MM, Fan M, Abdel-Rahman AA
Alcohol (Fayetteville, N.Y.) 2012 Dec;46(8):727-35

Sex differences in nitrosative stress during renal ischemia.

Rodríguez F, Nieto-Cerón S, Fenoy FJ, López B, Hernández I, Martinez RR, Soriano MJ, Salom MG
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Nov;299(5):R1387-95

Phosphorylation of N-methyl-D-aspartic acid receptor-associated neuronal nitric oxide synthase depends on estrogens and modulates hypothalamic nitric oxide production during the ovarian cycle.

Parkash J, d'Anglemont de Tassigny X, Bellefontaine N, Campagne C, Mazure D, Buée-Scherrer V, Prevot V
Endocrinology 2010 Jun;151(6):2723-35

Nitric oxide synthase in cardiac sarcoplasmic reticulum.

Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-nNOS pSer1417 was performed by loading 20 µg untreated SH-SY5Y cells (lane 1) and SH-SY5Y cells treated with 100 ng/mL nocodazole (lane 2) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Phospho-nNOS pSer1417 polyclonal antibody (Product # PA1-032) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~161 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Phospho-nNOS pSer1417 (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/mL nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-nNOS pSer1417 polyclonal antibody (Product # PA1-032) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.