SP5403

antibody from Acris Antibodies GmbH

Targeting: NOS1 nNOS, NOS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

SP5403

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SP5403, RRID:AB_1001072

Product name

anti NOS1

Antibody type

Polyclonal

Antigen

Synthetic Peptide corresponding to amino acid residues 724-739 of Rat bNOS.

Reactivity

Mouse, Rat, Bovine, Rabbit

Host

Rabbit

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of nNOS was performed by loading 40 µg of Mouse (Lane 1) and Rat Brain (Lane 2) tissue lysate onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a Nitrocellulose membrane. Membranes were probed with a Rabbit polyclonal antibody Cat.-No SP5403 recognizing nNOS at a dilution of 1/1000. The western blot was performed using the Fast Western Kit supersignal West Dura.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with Cat.-No SP5403 or without primary antibody (negative control) overnight at 4°C in a humidified chamber.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry was performed on normal biopsies of deparaffinized mouse skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/400 with Cat.-No SP5403 or without primary antibody (negative control) overnight at 4°C in a humidified chamber.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical staining of bNOS in rat brain using SP5403 antibody.