NB100-858

Brokhman I, Xu J, Coles BLK, Razavi R, Engert S, Lickert H, Babona-Pilipos R, Morshead CM, Sibley E, Chen C, van der Kooy D
Developmental biology 2019 Jan 15;445(2):256-270

Expression of RAD21 immunoreactivity in myenteric neurons of the human and mouse small intestine.

Bianco F, Eisenman ST, Colmenares Aguilar MG, Bonora E, Clavenzani P, Linden DR, De Giorgio R, Farrugia G, Gibbons SJ
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2018 Sep;30(9):e13429

Electrophysiological and morphological changes in colonic myenteric neurons from chemotherapy-treated patients: a pilot study.

Carbone SE, Jovanovska V, Brookes SJ, Nurgali K
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2016 Jul;28(7):975-84

Neuroprotective Potential of Mesenchymal Stem Cell-Based Therapy in Acute Stages of TNBS-Induced Colitis in Guinea-Pigs.

Robinson AM, Miller S, Payne N, Boyd R, Sakkal S, Nurgali K
PloS one 2015;10(9):e0139023

Myenteric expression of nerve growth factor and the p75 neurotrophin receptor regulate axonal remodeling as a consequence of colonic inflammation in mice.

Petrie CN, Armitage MN, Kawaja MD
Experimental neurology 2015 Sep;271:228-40

Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon.

Pelayo JC, Veldhuis NA, Eriksson EM, Bunnett NW, Poole DP
Cell and tissue research 2014 May;356(2):319-32

Effects of oxaliplatin on mouse myenteric neurons and colonic motility.

Wafai L, Taher M, Jovanovska V, Bornstein JC, Dass CR, Nurgali K
Frontiers in neuroscience 2013;7:30

Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons.

Zhao P, Canals M, Murphy JE, Klingler D, Eriksson EM, Pelayo JC, Hardt M, Bunnett NW, Poole DP
The Journal of biological chemistry 2013 Sep 6;288(36):25689-700

Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice.

Fried HU, Kaupp UB, Müller F
Cell and tissue research 2010 Mar;339(3):463-79

Expression and function of the bile acid receptor GpBAR1 (TGR5) in the murine enteric nervous system.

Poole DP, Godfrey C, Cattaruzza F, Cottrell GS, Kirkland JG, Pelayo JC, Bunnett NW, Corvera CU
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2010 Jul;22(7):814-25, e227-8

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: nNOS Antibody [NB100-858] - (A) Human Frontal Cortex (2 ug/mL) and (B) Brain Sub Ventricular Zone (1 ug/mL) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing nuclear and Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Frozen: nNOS Antibody [NB100-858] - Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse hypothalamus. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Eva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: nNOS Antibody [NB100-858] - Paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary antibody incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.