PA5-17706

antibody from Invitrogen Antibodies

Targeting: NOS3 ECNOS, eNOS

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17706

Provider

Invitrogen Antibodies

Product name

Phospho-eNOS (Thr495) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

44 µg/mL

Storage

-20°C

References

Submitted references

Global REACH 2018: dysfunctional extracellular microvesicles in Andean highlander males with excessive erythrocytosis.

Brewster LM, Bain AR, Garcia VP, Fandl HK, Stone R, DeSouza NM, Greiner JJ, Tymko MM, Vizcardo-Galindo GA, Figueroa-Mujica RJ, Villafuerte FC, Ainslie PN, DeSouza CA
American journal of physiology. Heart and circulatory physiology 2021 May 1;320(5):H1851-H1861

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Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats.

Fan Y, Fan H, Zhu B, Zhou Y, Liu Q, Li P
BMC complementary and alternative medicine 2019 Dec 5;19(1):355

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Widespread Coronary Dysfunction in the Absence of HDL Receptor SR-B1 in an Ischemic Cardiomyopathy Mouse Model.

Pearson JT, Yoshimoto M, Chen YC, Sultani R, Edgley AJ, Nakaoka H, Nishida M, Umetani K, Waddingham MT, Jin HL, Zhang Y, Kelly DJ, Schwenke DO, Inagaki T, Tsuchimochi H, Komuro I, Yamashita S, Shirai M
Scientific reports 2017 Dec 22;7(1):18108

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 AS-IV reversed high glucose-induced endothelial nitric oxide synthase (eNOS) Serum NO ( a ) and Renal Tissue NO ( b ) after AS-IV treatment in rats were detected by Nitric Oxide (NO) assay kit. n = 6 per group. Results are represented as mean and SD. c representative blot image of acetylated eNOS. d Western blot analysis for the expressions of eNOS, p-eNOS (Ser1177) and p-eNOS (Thr495). **** p < 0.0001 vs. control; ## p < 0.01 vs. DN. & p < 0.05

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 AS-IV reversed HG-induced eNOS impairment in HRGECs. a The NO producing ability in HRGECs after high glucose treatment was assessed by Nitric Oxide (NO) assay kit. All results were expressed as mean +- SD ( n = 3). b The expressions of eNOS, p-eNOS (Ser1177) and p-eNOS (Thr495) after high glucose treatment in HRGECs were detected by western blot. **** p < 0.0001, *** p < 0.001 vs. control; ### p < 0.001, ## p < 0.01 vs. HG; %% p < 0.01 vs HG + AS-IV