PA5-17917

antibody from Invitrogen Antibodies

Targeting: NOS3 ECNOS, eNOS

Western blot (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17917

Provider

Invitrogen Antibodies

Product name

Phospho-eNOS (Ser1177) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

79 µg/mL

Storage

-20°C

References

Submitted references

Artemisinin and Its Derivate Alleviate Pulmonary Hypertension and Vasoconstriction in Rodent Models.

Bao C, He Q, Wang H, Sun Y, Xu Y, Pan Y, Hu Y, Zheng S, Liang S, Luo A, Nahar T, Chen J, Tang H, Han Y
Oxidative medicine and cellular longevity 2022;2022:2782429

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Blood pressure-independent inhibition of Marfan aortic root widening by the angiotensin II receptor blocker valsartan.

Tehrani AY, White Z, Milad N, Esfandiarei M, Seidman MA, Bernatchez P
Physiological reports 2021 May;9(10):e14877

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Serelaxin activates eNOS, suppresses inflammation, attenuates developmental delay and improves cognitive functions of neonatal rats after germinal matrix hemorrhage.

Xu MM, Seyler L, Bäuerle T, Kalinichenko LS, Müller CP, Huttner HB, Schwab S, Manaenko A
Scientific reports 2020 May 15;10(1):8115

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Neurogranin regulates eNOS function and endothelial activation.

Cheriyan VT, Alfaidi M, Jorgensen AN, Alam MA, Abdullah CS, Kolluru GK, Bhuiyan MS, Kevil CG, Orr AW, Nam HW
Redox biology 2020 Jul;34:101487

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Perfluorooctane sulfonic acid (PFOS) exposure during pregnancy increases blood pressure and impairs vascular relaxation mechanisms in the adult offspring.

Dangudubiyyam SV, Mishra JS, Zhao H, Kumar S
Reproductive toxicology (Elmsford, N.Y.) 2020 Dec;98:165-173

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Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.

Waddingham MT, Sonobe T, Tsuchimochi H, Edgley AJ, Sukumaran V, Chen YC, Hansra SS, Schwenke DO, Umetani K, Aoyama K, Yagi N, Kelly DJ, Gaderi S, Herwig M, Kolijn D, Mügge A, Paulus WJ, Ogo T, Shirai M, Hamdani N, Pearson JT
Journal of molecular and cellular cardiology 2019 Dec;137:119-131

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Widespread Coronary Dysfunction in the Absence of HDL Receptor SR-B1 in an Ischemic Cardiomyopathy Mouse Model.

Pearson JT, Yoshimoto M, Chen YC, Sultani R, Edgley AJ, Nakaoka H, Nishida M, Umetani K, Waddingham MT, Jin HL, Zhang Y, Kelly DJ, Schwenke DO, Inagaki T, Tsuchimochi H, Komuro I, Yamashita S, Shirai M
Scientific reports 2017 Dec 22;7(1):18108

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Role of aromatase in sex-specific cerebrovascular endothelial function in mice.

Zuloaga KL, Davis CM, Zhang W, Alkayed NJ
American journal of physiology. Heart and circulatory physiology 2014 Apr 1;306(7):H929-37

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Methylene blue modulates transendothelial migration of peripheral blood cells.

Werner I, Guo F, Bogert NV, Stock UA, Meybohm P, Moritz A, Beiras-Fernandez A
PloS one 2013;8(12):e82214

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Effects of Serelaxin on post-GMH brain inflammation. ( A ) Representative western blot results. 72 hours after GMH significant decrease of e-NOS phosphorylation in brain of none treated compared to sham operated animals was observed ( B ). Consequently, overproduction of inflammatory factors IL-6 and TNFalpha in the brain was induced ( C , D respectively). Serelaxin reversed GMH effect on e-NOS phosphorylation, decreasing brain inflammation after GMH. *p < 0.05 compared to sham, #p < 0.05 compared to GMH + vehicle, N = 8/group. Data are mean +- SEM. ANOVA, Dunnett.