Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX42866 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX42866, RRID:AB_11166346
- Product name
- eNOS (phospho Ser1177) antibody [BDI250]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) Bend 3 cells were separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 1μg/ml GTX42866 for 1 hour at 15-22°C and developed by ECL (exp. time: 3 min). GTX42866 recognizes eNOS pS 1177 at 140 kDa.
