bs-3731R

antibody from Bioss

Targeting: NOS3 ECNOS, eNOS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-3731R

Provider

Bioss

Proper citation

Bioss Cat#bs-3731R, RRID:AB_10857653

Product name

eNOS (Thr495) Polyclonal Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

L1 mouse liver lysate L2 mouse kidney lysates probed with Rabbit Anti-Phospho-eNOS (Thr495) Polyclonal Antibody, Unconjugated (bs-3731R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 133kD. Observed band size: 133kD

Sample type

Mouse

Other comments

Kidney

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human kidney labeled with Anti-Phospho-eNOS (Thr495) Polyclonal Antibody, Unconjugated (bs-3731R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Human

Other comments

Kidney