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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- AF950 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal eNOS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human eNOS in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells.
High glutathionylation of placental endothelial nitric oxide synthase in preeclampsia.
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.
Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells.
Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compression.
Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
Wang Z, Wang F, Kong X, Gao X, Gu Y, Zhang J
Mediators of inflammation 2019;2019:7162976
Mediators of inflammation 2019;2019:7162976
High glutathionylation of placental endothelial nitric oxide synthase in preeclampsia.
Guerby P, Swiader A, Augé N, Parant O, Vayssière C, Uchida K, Salvayre R, Negre-Salvayre A
Redox biology 2019 Apr;22:101126
Redox biology 2019 Apr;22:101126
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H
Molecular medicine reports 2018 Aug;18(2):1987-1994
Molecular medicine reports 2018 Aug;18(2):1987-1994
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.
Liu Y, Huang Y, Xu Y, Qu P, Wang M
IUBMB life 2018 Apr;70(4):336-343
IUBMB life 2018 Apr;70(4):336-343
Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells.
Kawai Y, Yokoyama Y, Kaidoh M, Ohhashi T
American journal of physiology. Cell physiology 2010 Mar;298(3):C647-55
American journal of physiology. Cell physiology 2010 Mar;298(3):C647-55
Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compression.
Hennerbichler A, Fermor B, Hennerbichler D, Weinberg JB, Guilak F
Biochemical and biophysical research communications 2007 Jul 13;358(4):1047-53
Biochemical and biophysical research communications 2007 Jul 13;358(4):1047-53
Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
Wright JL, Tai H, Churg A
American journal of respiratory cell and molecular biology 2004 Nov;31(5):501-9
American journal of respiratory cell and molecular biology 2004 Nov;31(5):501-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human eNOS by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human eNOS. eNOS was immunoprecipitated from lysates (1 x 106 cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation.
